corecture F_CPU and osccal, there seems to be a bug only when connected to the pc but not to the wall adapter

makefile

@@ -10,7 +10,7 @@ FORMAT = ihex
 TARGET = main
 SRC = $(TARGET).c avr_adc.c fix_fft.c light_ws2812_AVR/Light_WS2812/light_ws2812.c 
 ASRC = 
-OPT = 0
+OPT = 1
 
 # Name of this Makefile (used for "make depend").
 MAKEFILE = Makefile
@@ -18,7 +18,7 @@ MAKEFILE = Makefile
 # Debugging format.
 # Native formats for AVR-GCC's -g are stabs [default], or dwarf-2.
 # AVR (extended) COFF requires stabs, plus an avr-objcopy run.
-DEBUG = stabs
+#DEBUG = stabs
 
 # Compiler flag to set the C Standard level.
 # c89   - "ANSI" C
@@ -28,7 +28,7 @@ DEBUG = stabs
 CSTANDARD = -std=gnu99
 
 # Place -D or -U options here
-CDEFS = -D F_CPU=32000000UL
+CDEFS = -D F_CPU=16000000UL
 
 # Place -I options here
 CINCS =
@@ -38,7 +38,7 @@ CDEBUG = -g$(DEBUG)
 CWARN = -Wall -Wstrict-prototypes
 CTUNING = -funsigned-char -funsigned-bitfields -fpack-struct -fshort-enums
 #CEXTRA = -Wa,-adhlns=$(<:.c=.lst)
-CFLAGS = $(CDEBUG) $(CDEFS) $(CINCS) -O$(OPT) $(CWARN) $(CSTANDARD) $(CEXTRA)
+CFLAGS = $(CDEBUG) $(CDEFS) $(CINCS) -O$(OPT) $(CWARN) $(CSTANDARD)# $(CEXTRA)
 
 
 #ASFLAGS = -Wa,-adhlns=$(<:.S=.lst),-gstabs